Part2:Fluorometricmethodforcheese
Thisstandardspecifiesafluorometricmethodforthedeterminationofalkalinephosphataseactivityincheese.
Thismethodisalsoapplicabletosoftcheesesandsemi-hardcheesesfordistinguishingrawmilkcheesesfromcheeseproducedwithpasteurizedmilk,providedthatthemouldisonlyonthesurfaceandnotalsointheinnerpart(e.g.blue-veinedcheeses).Thismethodcanalsobeusedtochecktheproperpasteurizationofcheeseoritsrawmaterial.
Inlargehardcheeseswherethewheycurdmixtureisscaldedattemperaturesabove50degreesCelsius,hightemperaturesremainforarelativelylongtime.Thisisespeciallythecaseinthecentreofthesecheeses,thuspromotingphosphataseinactivation.Todistinguish,therefore,betweenhardcheesesfromrawmilkandhardcheesesfrompasteurizedmilkisdifficultandoftenevenimpossiblebythismethod.
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